4QQY

Crystal structure of T. fusca Cas3-ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	87.206, 222.607, 124.847
Unit cell angles	90.00, 104.07, 90.00

Refinement procedure

Resolution	47.836 - 3.120
R-factor	0.1713
Rwork	0.170
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.474
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.120	3.120
Rmerge	0.110
Number of reflections	81891
<I/σ(I)>	9.3
Completeness [%]	99.2	98.3
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K