4QQW

Crystal structure of T. fusca Cas3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-28
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	85.789, 218.542, 123.749
Unit cell angles	90.00, 105.00, 90.00

Refinement procedure

Resolution	30.207 - 2.664
R-factor	0.174
Rwork	0.173
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.410
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.058	0.378
Number of reflections	115323
<I/σ(I)>	18.1	1.9
Completeness [%]	92.7	86.8
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K