4QQK

Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae) with GMS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	I 41
Unit cell lengths	95.211, 95.211, 108.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.520 - 1.880
R-factor	0.1775
Rwork	0.176
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hc4
RMSD bond length	0.010
RMSD bond angle	1.361
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.520	50.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmerge	0.060	0.027	0.704
Number of reflections	39024
<I/σ(I)>	10.7
Completeness [%]	100.0	99.1	100
Redundancy	38.2	8.1	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 3350, 0.2 M KSCN, vapor diffusion hanging drop, temperature 291K