4QQJ
Crystal Structure of FGF Receptor (FGFR) 4 Kinase Domain Harboring the V550L Gate-Keeper Mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.671, 61.472, 61.819 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 43.293 - 1.682 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2psq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.972 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.680 |
| Rmerge | 0.052 |
| Number of reflections | 34654 |
| <I/σ(I)> | 50.2 |
| Completeness [%] | 96.6 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Tris (pH 7.5), 20% (w/v) PEG 1500 and 0.2 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
