4QQD
Crystal Structure of tandem tudor domains of UHRF1 in complex with a small organic molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-13 |
| Detector | adsc q315 |
| Wavelength(s) | 0.9791831 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.338, 87.338, 83.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.280 |
| R-factor | 0.2038 |
| Rwork | 0.200 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished structure of UHRF1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.5) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.670 | 43.670 | 2.360 |
| High resolution limit [Å] | 2.280 | 8.830 | 2.280 |
| Rmerge | 0.093 | 0.029 | 0.944 |
| Total number of observations | 3030 | 18654 | |
| Number of reflections | 16676 | ||
| <I/σ(I)> | 21.9 | 63.4 | 2.9 |
| Completeness [%] | 100.0 | 98.3 | 100 |
| Redundancy | 11.3 | 10 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 20% PEG-3350, 0.2 M magnesium nitrate, vapor diffusion, sitting drop |
