4QPZ
Crystal structure of the formolase FLS_v2 in space group P 21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.878, 136.564, 167.062 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 166.330 - 3.000 |
R-factor | 0.21022 |
Rwork | 0.208 |
R-free | 0.25658 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ago |
RMSD bond length | 0.010 |
RMSD bond angle | 1.510 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (MR) |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 166.330 | 3.230 | 3.110 |
High resolution limit [Å] | 3.000 | 3.110 | 3.000 |
Number of reflections | 89485 | ||
<I/σ(I)> | 10.1 | 2.96 | 2.01 |
Completeness [%] | 91.0 | 86 | 85.4 |
Redundancy | 3.5 | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG3350, 100 mM Succinic Acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |