4QO6
Structural studies of CdsD, a structural protein of the Type III secretion system (TTSS) of Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.600, 23.910, 118.650 |
| Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
| Resolution | 28.660 - 2.260 |
| R-factor | 0.19696 |
| Rwork | 0.195 |
| R-free | 0.24133 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.766 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (AutoSolve) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.660 | 2.390 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 13969 | |
| Completeness [%] | 97.6 | 92.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 50 mM MES; pH 6.0 - 3 % PEG4000 - 15 % 2-propanol - 50 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
