4QNN

Crystal Structure of phospholipase A 1 from hornet(Vespa basalis) venom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL17B2
Synchrotron site	NSRRC
Beamline	BL17B2
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2004-05-07
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	57.276, 70.147, 81.724
Unit cell angles	107.14, 109.99, 100.90

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.2042
Rwork	0.204
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Number of reflections	35849
Completeness [%]	96.5	97.2	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.2M sodium acetate trihydrate, 0.1M Tris HCl pH 8.0, 25% PEG4000 500ml precipitation, VAPOR DIFFUSION, SITTING DROP, temperature 298K