4QMA
Crystal Structure of a Putative Cysteine Dioxygnase From Ralstonia eutropha: An Alternative Modeling of 2GM6 from JCSG Target 361076
Replaces: 2GM6Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-13 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.007, 57.007, 216.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.748 - 1.650 |
R-factor | 0.1754 |
Rwork | 0.174 |
R-free | 0.20000 |
Starting model (for MR) | Fourier Synthesis based on 2GM6 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.213 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.113 | 0.533 |
Number of reflections | 44377 | |
<I/σ(I)> | 14.1 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21 | 16.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 2.4M (NH4)2SO4, 0.1M TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 2.4M (NH4)2SO4, 0.1M TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |