4QLN
structure of ydao riboswitch binding with c-di-dAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.874, 87.051, 144.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.250 - 2.650 |
R-factor | 0.221 |
Rwork | 0.217 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.820 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.650 |
Number of reflections | 49287 |
Completeness [%] | 1.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | 0.1 M Na-Bicine, pH 8.7-9.2 and 2.6-3.2 M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |