4QL7
Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 137.129, 137.129, 145.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.750 |
| R-factor | 0.274 |
| Rwork | 0.273 |
| R-free | 0.28980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.747 |
| Data reduction software | MOSFLM (3.3.20) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.950 |
| High resolution limit [Å] | 3.750 | 3.750 |
| Rmerge | 0.058 | 0.505 |
| Number of reflections | 16581 | |
| <I/σ(I)> | 14.6 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.8M ammonium sulfate, 100mM MES, pH 6.5, 10mM cobalt chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
