4QJF

X-ray crystal structure of Thermocuccus kodakarensis RNA polymerase Rpp4/Rpo7 (RpoF/RpoE) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-12
Detector	RIGAKU SATURN 944+
Wavelength(s)	0.9714
Spacegroup name	P 21 21 21
Unit cell lengths	42.465, 80.942, 97.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.794 - 2.312
R-factor	0.1963
Rwork	0.193
R-free	0.26080
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1626))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	12575
Completeness [%]	86.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	300	0.1 M NaAcetate and 30 % glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K