4QID
Crystal structure of Haloquadratum walsbyi bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.939, 29.799, 124.973 |
| Unit cell angles | 90.00, 118.76, 90.00 |
Refinement procedure
| Resolution | 28.870 - 2.570 |
| R-factor | 0.20818 |
| Rwork | 0.207 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.570 | 2.580 |
| Rmerge | 0.168 | 0.791 |
| Number of reflections | 48309 | |
| <I/σ(I)> | 8.212 | 2.016 |
| Completeness [%] | 97.8 | 98 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPID CUBIC PHASE | 4 | 293 | ammonium sulfate, NaCl, sodium acetate, PEG 200, pH 4.0, LIPID CUBIC PHASE, temperature 293.0K |
