4QI7

Cellobiose dehydrogenase from Neurospora crassa, NcCDH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-20
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	133.599, 141.851, 146.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.040 - 2.900
R-factor	0.187
Rwork	0.185
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.3_1477))

Data quality characteristics

	Overall
Low resolution limit [Å]	58.640
High resolution limit [Å]	2.900
Number of reflections	62494
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M Mes-OH pH 6.5, 1.5 M magnesium sulfate, 0.02 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K