4QI1
Crystal structure of H. walsbyi bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.697, 61.634, 119.512 |
| Unit cell angles | 90.00, 116.17, 90.00 |
Refinement procedure
| Resolution | 26.730 - 1.850 |
| R-factor | 0.19922 |
| Rwork | 0.197 |
| R-free | 0.23738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.848 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.117 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | |
| Number of reflections | 74113 | |
| <I/σ(I)> | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPID CUBIC PHASE | 5.5 | 293 | sodium citrate, NaCl, PEG 400, pH 5.5, LIPID CUBIC PHASE, temperature 293.0K |
