4QH4

The GLIC pentameric Ligand-Gated Ion Channel (wild-type) crystallized in acetate buffer at pH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2012-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	180.800, 133.383, 159.613
Unit cell angles	90.00, 102.16, 90.00

Refinement procedure

Resolution	20.000 - 3.200
R-factor	0.202
Rwork	0.201
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	3.370
High resolution limit [Å]	3.200	3.200
Number of reflections	60657
Completeness [%]	99.6	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	400 mM Sodium Isothiocyanate 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000, 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K