4QGO
Crystal structure of NucA from Streptococcus agalactiae with no metal bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 37.591, 57.131, 62.422 |
Unit cell angles | 109.40, 97.08, 90.16 |
Refinement procedure
Resolution | 30.555 - 1.500 |
R-factor | 0.154 |
Rwork | 0.153 |
R-free | 0.18030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3owa |
RMSD bond length | 0.006 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 74966 | |
<I/σ(I)> | 10.7 | |
Completeness [%] | 96.3 | 91.4 |
Redundancy | 3.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.6M ammonium sulfate, 0.1M sodium citrate and 0.2M NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |