4QGK
Structure of the Human Sjogren Larsson Syndrome enzyme fatty aldehyde dehydrogenase (FALDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.137, 98.582, 145.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.19962 |
| Rwork | 0.198 |
| R-free | 0.22257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sza |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 66111 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 298 | 0.2M Li2SO4, 0.1M CHES pH9.5, 1.0M K/Na Tartrate, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 9.5 | 298 | 0.2M Li2SO4, 0.1M CHES pH9.5, 1.0M K/Na Tartrate, VAPOR DIFFUSION, temperature 298K |
