4QF2

Crystal structure of human BAZ2A PHD zinc finger in the free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2822
Spacegroup name	P 43 21 2
Unit cell lengths	72.014, 72.014, 99.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.250 - 1.700
R-factor	0.18383
Rwork	0.182
R-free	0.21502
Structure solution method	SAD
Starting model (for MR)	Arp/wArp autobuilding model
RMSD bond length	0.012
RMSD bond angle	1.926
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.250	29.120	1.790
High resolution limit [Å]	1.700	5.370	1.700
Rmerge	0.063	0.046	0.514
Number of reflections	27299
<I/σ(I)>	24.9	45.6	5.9
Completeness [%]	100.0	100	100
Redundancy	14.8	7.7	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2M Na/K phosphate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K