4QEQ
High resolution structure of egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.650, 77.650, 37.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.536 - 1.381 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.18250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.560 | 1.460 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.048 | 0.350 |
| Number of reflections | 23732 | |
| <I/σ(I)> | 21 | 5.1 |
| Completeness [%] | 80.0 | 100 |
| Redundancy | 7.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 9% w/v sodium chloride, 0.1 M sodium acetate, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
