4QDY

Crystal structure of a YbbR-like protein (SP_1560) from Streptococcus pneumoniae TIGR4 at 2.74 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97966,0.97895
Spacegroup name	P 64 2 2
Unit cell lengths	77.930, 77.930, 145.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.330 - 2.740
R-factor	0.2346
Rwork	0.232
R-free	0.28780
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.330		2.840
High resolution limit [Å]	2.740	5.860	2.740
Rmerge	0.139	0.013	0.013
Number of reflections	7389	858	727
<I/σ(I)>	18.36	45.7	2.7
Completeness [%]	99.7	98.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	42.0% polyethylene glycol 1500, 0.2M ammonium sulfate, 0.1M cesium chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K