4QDV
Dcps in complex with covalent ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.090, 105.510, 140.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.260 - 2.800 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.24990 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.260 | 3.130 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.170 | 0.910 |
| Number of reflections | 35486 | |
| <I/σ(I)> | 12 | 4.1 |
| Completeness [%] | 95.3 | 97.3 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 22-25% PEG3350, 50 mM sodium chloride, 26% glycerol, crystals soaked in 1 mM compound overnight, VAPOR DIFFUSION, HANGING DROP |
