4QCI
PDGF-B blocking antibody bound to PDGF-BB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2012-06-01 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.150, 68.470, 95.250 |
| Unit cell angles | 90.00, 97.56, 90.00 |
Refinement procedure
| Resolution | 69.640 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.000 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.640 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 51112 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 18 C from a solution containing 22% PEG 3350 and 0.1M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
