4QC8
Structural annotation of pathogenic bovine Parvovirus-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 323.450, 381.230, 376.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.310 |
| R-free | 0.31150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s58 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.722 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
| Rmerge | 0.124 | ||
| Number of reflections | 340823 | ||
| <I/σ(I)> | 7.6 | 1.8 | |
| Completeness [%] | 90.1 | 81.4 | 93.2 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10 mM Tris, 4% PEG8000, 8 mM magnesium chloride, 50 mM lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
