4QC4

Crystal structure of C117S mutant of human acidic fibroblast growth factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97911
Spacegroup name	C 2 2 21
Unit cell lengths	73.493, 95.223, 106.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.422 - 1.491
R-factor	0.1622
Rwork	0.162
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.259
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser MR))
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.180
High resolution limit [Å]	1.491	1.491
Number of reflections	60567
Completeness [%]	99.9
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.9 M sodium citrate, 0.1 M imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K