4QBU
Structure of the Acyl Transferase domain of ZmaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.915, 74.250, 124.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.510 - 1.700 |
| R-factor | 0.17257 |
| Rwork | 0.171 |
| R-free | 0.20046 |
| Structure solution method | SAD + molecular replacement |
| Starting model (for MR) | 2qo3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.706 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 54382 | |
| <I/σ(I)> | 27.7 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | mother liquor 200 mM MgCl2, 20% PEG 4000, 800 mM sodium formate, 100 mM BisTris; drops equal volume 7 mg/ml protein and mother liquor, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
