4QBM

Crystal structure of human BAZ2A bromodomain in complex with a diacetylated histone 4 peptide (H4K16acK20ac)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 41
Unit cell lengths	64.869, 64.869, 71.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.870 - 1.650
R-factor	0.18631
Rwork	0.185
R-free	0.20495
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lz2 BAZ2A
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.010
High resolution limit [Å]	1.650
Rmerge	0.067
Number of reflections	35633
<I/σ(I)>	15.6
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1M sodium phosphate monobasic, 0.1M potassium phosphate monobasic, 2 M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K