4QBC
Crystal structure of C117T mutant of human acidic fibroblast growth factor in sodium formate buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.735, 95.377, 106.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.266 - 1.519 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.172 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((Phaser MR)) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.519 | 1.519 |
| Number of reflections | 57173 | |
| Completeness [%] | 98.1 | |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4.0 M sodium formate, 0.45 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
