4QAI
P. stipitis OYE2.6-Y78W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.541 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 226.787, 226.787, 360.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.650 - 2.750 |
| Rwork | 0.224 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | OYE 2.6 Y78W I113C |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.650 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 92137 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 3M sodium malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
