4Q9R
Crystal structure of an RNA aptamer bound to trifluoroethyl-ligand analog in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-04-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.515, 78.986, 94.357 |
Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
Resolution | 67.680 - 3.120 |
R-factor | 0.2056 |
Rwork | 0.203 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.545 |
Data reduction software | RAPD |
Data scaling software | RAPD |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.680 | 3.290 |
High resolution limit [Å] | 3.120 | 3.120 |
Number of reflections | 17701 | |
Completeness [%] | 98.1 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% Tacsimate pH 7.0, 20% PEG 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |