4Q9G
Crystal structure of K12V/C16S/C117V/P134V mutant of human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.020 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.395, 97.327, 108.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.180 - 1.554 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.761 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((Phaser MR)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 55669 | |
Completeness [%] | 97.0 | 90.5 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.8 M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |