4Q93
Crystal structure of resveratrol bound human tyrosyl tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.966, 162.550, 35.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.010 - 2.100 |
| R-factor | 0.17723 |
| Rwork | 0.174 |
| R-free | 0.23071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n3l |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | 0.271 |
| Number of reflections | 25438 | |
| <I/σ(I)> | 28.5 | 4.1 |
| Completeness [%] | 99.0 | 93.2 |
| Redundancy | 4.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2.1 M ammonium sulfate, 0.1 M sodium phosphate monobasic / potassium phosphate dibasic, pH 6-8, 2% acetone, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
