4Q91
Crystal structure of C16A/K12V/C117V/P134V mutant of human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.138, 97.104, 108.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.130 - 1.799 |
| R-factor | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.927 | |
| High resolution limit [Å] | 1.799 | 1.799 |
| Number of reflections | 36344 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.4 M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
