4Q7Y

Neutrophil serine protease 4 (PRSS57) apo form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	110
Detector technology	CCD
Collection date	2012-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977408
Spacegroup name	P 63
Unit cell lengths	86.636, 86.636, 69.351
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.320 - 2.700
R-factor	0.1928
Rwork	0.187
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hne
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	8192
<I/σ(I)>	12.8
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	23% PEG3350, 0.2 M NaCl, 0.1 M Bis-Tris 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K