4Q7U
Crystal structure of photoswitchable fluorescent protein PSmOrange2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.724, 43.874, 52.544 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 52.390 - 1.300 |
| R-factor | 0.1494 |
| Rwork | 0.148 |
| R-free | 0.17670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.391 | 52.391 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.050 | 0.026 | 0.361 |
| Number of reflections | 49302 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 96.4 | 98.8 | 67.5 |
| Redundancy | 3.4 | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, PEG1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
