4Q7R
Crystal structure of large Stokes shift fluorescent protein LSSmOrange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.436, 107.364, 56.602 |
| Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
| Resolution | 55.330 - 1.400 |
| R-factor | 0.1479 |
| Rwork | 0.147 |
| R-free | 0.17480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.977 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.335 | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.056 | 0.034 | 0.628 |
| Number of reflections | 84546 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 98.6 | 99.7 | 97.1 |
| Redundancy | 4.2 | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.2 M zinc acetate, 0.1 M acetate buffer, 20% PEG1000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
