4Q7Q
The crystal structure of a possible lipase from Chitinophaga pinensis DSM 2588
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 43 |
| Unit cell lengths | 80.611, 80.611, 93.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.200 - 1.451 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18970 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.091 | 0.628 |
| Number of reflections | 104383 | |
| <I/σ(I)> | 46.3 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.7 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 289 | 0.1M CHES:NaOH, 30% (v/v) PEG 400, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
