4Q7N
Crystal structure of the complex of Buffalo Signalling protein SPB-40 with 4-N-trimethylaminobutyraldehyde at 1.79 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-11-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.068, 66.735, 106.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.490 - 1.790 |
| R-factor | 0.18698 |
| Rwork | 0.185 |
| R-free | 0.22941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o9o |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.458 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 40939 | |
| <I/σ(I)> | 27.7 | 2.1 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
