4Q6F
Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3K4 histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.866, 71.866, 98.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.060 - 1.910 |
| R-factor | 0.18472 |
| Rwork | 0.183 |
| R-free | 0.21864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.553 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.060 |
| High resolution limit [Å] | 1.910 |
| Rmerge | 0.062 |
| Number of reflections | 20562 |
| <I/σ(I)> | 20.4 |
| Completeness [%] | 100.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2M malic acid, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
