4Q65
Structure of the E. coli Peptide Transporter YbgH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 94.035, 109.448, 76.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.540 - 3.400 |
R-factor | 0.2603 |
Rwork | 0.258 |
R-free | 0.31570 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.273 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.700 |
High resolution limit [Å] | 3.400 | 7.100 | 3.400 |
Number of reflections | 9105 | ||
Completeness [%] | 72.0 | 88.4 | 45.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |