4Q5I
Crystal structure of a T151A mutant of the E. coli FeoB G-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.978, 82.779, 95.505 |
| Unit cell angles | 115.67, 108.50, 90.06 |
Refinement procedure
| Resolution | 45.060 - 2.800 |
| R-factor | 0.2415 |
| Rwork | 0.239 |
| R-free | 0.29829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.682 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.066 | 0.032 | 0.367 |
| Number of reflections | 45132 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 97.2 | 97.9 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 29% PEG 400, 0.1M HEPES pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
