4Q48
Structure of the RecQ Catalytic Core from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.791, 98.528, 152.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.312 - 2.797 |
| R-factor | 0.218 |
| Rwork | 0.213 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.577 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.797 | 2.800 |
| Rmerge | 0.543 | |
| Number of reflections | 31511 | |
| Completeness [%] | 96.3 | 96.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 0.1M HEPES (pH 7.7), 14% PEG 8K, 8% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
