4Q3I

Structure of the OsSERK2 leucine rich repeat extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	67.881, 50.646, 83.161
Unit cell angles	90.00, 113.25, 90.00

Refinement procedure

Resolution	42.215 - 2.350
R-factor	0.2411
Rwork	0.236
R-free	0.28580
RMSD bond length	0.002
RMSD bond angle	0.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (dev_1525)
Refinement software	PHENIX ((phenix.refine: dev_1616))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	22466
Completeness [%]	95.0	71.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	100 mM Bis-Tris pH 6.5, 200 mM MgCl2, and 23% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K