4Q3H
The crystal structure of NHERF1 PDZ2 CXCR2 complex revealed by the NHERF1 CXCR2 chimeric protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | .97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.751, 55.349, 45.226 |
| Unit cell angles | 90.00, 90.74, 90.00 |
Refinement procedure
| Resolution | 32.748 - 1.443 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.750 | 1.448 |
| High resolution limit [Å] | 1.443 | 1.443 |
| Rmerge | 0.042 | 0.369 |
| Number of reflections | 28239 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris HCl, pH 8.5, 8% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
