4Q37

Crystal structure of the hypothetical protein TM0182 Thermotoga maritima, N-terminal domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0698
Spacegroup name	C 2 2 21
Unit cell lengths	102.050, 145.300, 141.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.600 - 3.190
R-factor	0.2375
Rwork	0.235
R-free	0.28000
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.600	4.500	3.260
High resolution limit [Å]	3.190	4.300	3.190
Rmerge	0.014
Number of reflections	33708
<I/σ(I)>	15.01
Completeness [%]	99.1	100	88.5
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, 1.2 M Ammonium sulfate and 0.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K