4Q2T

Crystal structure of Arginyl-tRNA synthetase complexed with L-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Detector technology	CCD
Collection date	2013-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	74.485, 106.901, 175.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.910 - 2.400
R-factor	0.1981
Rwork	0.196
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bs2
RMSD bond length	0.009
RMSD bond angle	1.122
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.075	0.051	0.306
Number of reflections	55170
<I/σ(I)>	13.5
Completeness [%]	97.8	96.9	95.4
Redundancy	4.8	6.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	0.085mM Sodium citrate tribasic dehydrate, 24% PEG4000, 0.17M Ammonium acetate, 15% Glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K