4Q24
Crystal structure of Cyclo(L-leucyl-L-phenylalanyl) synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.984 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 86.968, 86.968, 70.738 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.660 - 2.900 |
R-factor | 0.2034 |
Rwork | 0.201 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oqv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.700 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 7023 | |
<I/σ(I)> | 11.45 | 1.75 |
Completeness [%] | 98.1 | 98.5 |
Redundancy | 2.71 | 2.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 278 | 10%PEG8000, 50mM KCl, 50mM Na cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |