4Q1S
Yeast 20S proteasome in Complex with Kendomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.440, 301.120, 145.540 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 48.525 - 2.600 |
| R-factor | 0.1779 |
| Rwork | 0.175 |
| R-free | 0.22369 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.525 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.485 |
| Number of reflections | 324230 | |
| <I/σ(I)> | 14.3 | 2.7 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 mM MgAc2, 100 mM MES, 13% MPD, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
