4Q0K
Crystal Structure of Phytohormone Binding Protein from Medicago truncatula in complex with gibberellic acid (GA3)
Replaces: 3US7Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-27 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91800 |
Spacegroup name | P 65 |
Unit cell lengths | 55.800, 55.800, 99.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.750 - 1.340 |
R-factor | 0.1238 |
Rwork | 0.123 |
R-free | 0.15720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2flh |
RMSD bond length | 0.019 |
RMSD bond angle | 1.651 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.750 | 4.070 | |
High resolution limit [Å] | 1.340 | 2.890 | |
Rmerge | 0.043 | 0.024 | |
Number of reflections | 38199 | 2553 | 1431 |
<I/σ(I)> | 30.64 | 96.92 | |
Completeness [%] | 97.8 | 100 | |
Redundancy | 10.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1 M ADA, 1.0 M AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |