4Q0I
Deinococcus radiodurans BphP PAS-GAF D207A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.497, 51.327, 80.368 |
| Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
| Resolution | 36.193 - 1.744 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.500 |
| High resolution limit [Å] | 1.744 |
| Number of reflections | 32523 |
| Completeness [%] | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 19% isopropano, 19% PEG 3350, 5% glycerol, 100 mM Citric acid(NaOH) pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
